Porcine 9 CFR Testing

Microtest offers expertise and reliable testing for Porcine parvovirus (PPV), both in trypsin derived from porcine pancreas and in biological samples previously exposed to trypsin (such as cell lines, bulk harvest, in-process samples, vaccines, etc). This testing is prescribed in the Code of Federal Regulations, Animals and Animal Products, Title 9 (9 CFR). Customers may also request the testing be modified for certain sample types, or extended, as desired.

The assay complies with 9 CFR, part 113.53. It tests for PPV that may have contaminated porcine pancreas derived Trypsin or other biological samples. An enzyme secreted by the pancreas, trypsin is widely used by laboratories in processing tissues for cell cultures. However, since during a pig’s life cycle it is highly susceptible to infection by PPV, a common swine disease, trypsin must always be checked for PPV contamination.

To determine whether trypsin samples or cell cultures exposed to trypsin are contaminated with PPV, they are inoculated into porcine cells susceptible to PPV, and maintained for at least 14 days. (This maintenance period may be extended upon request.) At least 7 days after inoculation, Microtest virologists prepare monolayers from the test cultures, and analyze them via immunofluoresence at end point using antibodies raised against PPV.

To serve as negative controls, cell cultures inoculated with culture medium are maintained in parallel with test cultures. Similarly, other monolayers are prepared from test cultures at least 7 days after inoculation; these positive controls are inoculated with PPV and likewise maintained in parallel.

As a final result, we report whether PPV is found in the tested porcine cells. Reporting time for porcine 9 CFR testing is 4 weeks. Call Microtest for information on standard protocols for test sample preparation, shipment, and packaging.