HPLC Assays

Microtest provides first-class results as a contract laboratory for high-performance liquid chromatography (HPLC) assays of pharmaceutical products. Rely on us to take charge of all kinds of specialized HPLC assay projects — from methods already completely validated to those run only once.

Microtest specialists can quickly identify potential problems in existing methods, and create smooth solutions. We also possess an enviable track record in developing and validating efficient methods of our own design. Our reverse phase HPLC procedures allow separation based on the polarity of the target molecule. Our size exclusion techniques depend on molecule size, while our ion exchange procedures are based on molecule charge.

Microtest performs the following analysis types:
• Assay
• Purity
• Impurities
• Content uniformity
• Identification
• Isocratic and gradient methods (for all types of analysis)

We have successfully analyzed the following sample types, among others:
• Small molecules
• Peptides
• Proteins
• Oligonucleotides
• Sample matrixes of
     — Solids
     — Liquids
     — Oils
     — Extractables

Expert Microtest chemists boast substantial experience in both R&D and contract laboratories. They use the most advanced equipment, including temperature-controlled autosamplers and column compartments, UV/VIS detectors, diode array detectors, and refractive Index detectors. In addition, we maintain four Agilent systems with Chemstation software, and a Shimadzu system with Class-VP software.